DNA

Part:BBa_K3304118:Design

Designed by: Sofia Moysiadou   Group: iGEM19_Thessaloniki   (2019-10-21)


Single stranded auxiliary strand for our ELK1 Input Gate - DTG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the correct designing of this DNA computing gate, the sequences must be produced and evaluated through our Genetic Algorithm model, while allowing for the inclusion of the recognition sites for both PvuII and Nb.BsrDI enzymes at the appropriate locations.


Source

The part is produced synthetically and can be inserted into bacterial plasmids through the Golden Gate assembly method. Its sequences are produced through our genetic algorithm model to be robust and orthogonal (https://2019.igem.org/Team:Thessaloniki/GeneticA).

References